1. 본 발명에 따른 재조합 아데노바이러스 벡터를 이용하여 Prnp 유전자를 Prnp 유전자가 녹-아웃된 마우스 신경세포주(Zpl 3-4)에 형질감염한 시스템은 정상 산소 상태에서 PrPC를 과발현시키고, 아넥신 V (+) 세포, 즉 아폽토시스가 일어난 세포의 비율과 LDH의 활성을 발현 카세트가 없는 재조합 아데노바이러스 벡터(Ad-lacZ)로 형질감염된 Zpl 3-4 세포에서보다 유의하게 감소시킨다.

In a system in which the Prnp gene is transfected into a mouse nerve cell strain (Zpl 3-4) from which the Prnp gene has been knocked out by using the recombinant adenovirus vector according to the present invention, in the normoxic state PrPC is over-expressed, and the proportion of annexin V (+) cells, which is to say cells where apoptosis has occurred, and the LDH activity are significantly reduced as compared with Zpl 3-4 cells transfected with a recombinant adenovirus vector (Ad-lacZ) having no expression cassette.